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With Jin's growing popularity, the Study of Medicine Office find him a threat to their livelihood and status as the medical authority. A surgical instrument of Saburi's is found in the room of a murdered prostitute. The doctors at the Study of Medicine Office use it to their advantage to accuse Saburi of murder. He confesses that he's had an agreement to do autopsies at the Yoshiwara in exchange for a proper burial for the prostitutes. Ogata takes responsibility and resigns. However, Jin decides it would be best if he resigned instead to keep Ogata as head of the ...

It is now winter of 1862. Jin had confessed to Saki that he is from the future. However, she has gone about as if nothing has happened. Kiichi is an apprentice at Daikichi's shop. Ryoma has become Katsu's student, while Kyotaro is his secretary. Jin is lecturing at the Western Medical office, with Saki in attendance and Ogata as his most fervent and out-spoken student. One day, as Jin is creating a tool that could drill a hole in a patient's skull, he and Saki get too close. She jumps away and asks him what he wants for dinner. She agrees to make him a special dish.

After the check-up with Oyoji, Courtesan Nokaze asks Jin to see another patient. He discovers a former courtesan in the last stages of syphilis. There is no cure, since penicillin has not yet been discovered. Jon tries to persuade the rest of the prostitutes at the brothel to accept to an examination for signs of syphilis. However, they call him a pervert and refuse. Feeling defeated for his inability to help Courtesan Nokaze, Jin suddenly recalls the first meeting with Miki. She presented him with the procedure to create penicillin. He immediately seeks the help of ...

Kyotaru sells his late father's treasured tea cups to buy a pair of glasses for Hatsune, a prostitute he first met while guarding Jin at Yoshiwara. With Ogata dead, Jin must prove his worthiness for Hamaguchi to fund making a more potent penicillin. Jin goes to Yoshiwara to see another ill prostitute, Hatsune. A local doctor performed an abortion that caused blood-poisoning. The penicillin is not potent enough. They must try to find immediate funding for a new production a much more potent dose. A male actor, who plays theatrical female roles, is asked to loan the ...

It is 1863. Jin recognizes that it is the beginning of the Bakumatsu period. Ryoma, (a prominent historical figure who fights for the creation of a modern government, to prevent colonization), has been sent to build a strong navy. Kiichi's mother, Tae, is severely burnt by hot oil. Jin is summoned and prepares to do a skin graft. He asks Ogata for lots of penicillin to prevent infection. Since the factory was destroyed, Yamada has been in charge of restarting the production. Ogata invites a friend, Hamaguchi, to observe the surgery. As the surgery is about to end, ...

Now that Jin has Koan Ogata, the supervisor of the Western Medicinal Office for the village, and his team on his side, Jin begins to create a large-scale plan. He asks if rubber hoses exist and if a syringe needle can be made to create an IV drip. The town begins to make saline solution, sterilize everything with alcohol, and burying waste and burnt clothes in large ditches. Ryoma takes it upon himself to be the leader of waste disposal. Jin teaches the doctors how to administer an IV drip. Kiichi and Yamada recover. Jin suddenly becomes sick and has delusions while ...

The restoration of imperial rule, which is Ryoma’s earnest wish for ‘Japan’s rebirth’, comes into effect. This brings about the end of 260 years of history of the shogunate. On the other hand, Jin recalls how Miki had previously said that Sakamoto Ryoma died on his birthday, and realises that they are just one month away from the day of the assassination. Ryoma’s assassination draws closer. Jin, who has decided that he will make sure he saves Ryoma with his own hands, sets off for Kyoto together with Saki and Saburi. Meanwhile, Kyotaro is ordered by his superior to do something … Jin and the rest try to meet Ryoma as soon as they can, but have a hard time locating him despite searching the streets of Kyoto. Then the day of Ryoma’s birthday, 15 November, comes.

Ryoma is finally slashed just like in the events in history. While he is watched over by Otose, the female proprietress of Teradaya, and others, Jin prepares himself for a major surgery with the help of Saki and Saburi.

Together with Saki, Jin frantically treats Ohatsu even as it seems like his own body is going to disappear. He vanishes before Saki’s eyes and in that moment, he sees something … Back in Edo, Jin’s struggle has been in vain. Ohatsu loses her life … and he is overcome by a feeling of hollowness. Jin returns to Jinyudo with Saki, where they learn from Katsu that Ryoma had been attacked at Teradaya. Painfully aware of his own powerlessness, Jin is alarmed by Ryoma’s looming assassination … Meanwhile, Tanosuke visits Jin at Jinyudo to entreat him to examine his senior apprentice, Bando Kichijuro, who has been bedridden as a result of illness. Because Kichijuro has Raynaud’s syndrome (a disorder of blood circulation in the fingers and toes which aggravates with exposure to the cold), his fingers and toes have become ulcerated and need to be amputated in order to prolong his life. However, Kichijuro wishes to stand on stage and perform a play one last time.

The an donuts that Jin and Saki had presented to Princess Kazunomiya are suspected to be laced with arsenic and they are put into the Kodenmacho jailhouse on charges of attempted poisoning. Because it is the princess who was poisoned, the matter has to be dealt with in absolute secrecy or it would jeopardise the union of the shogunate and the Imperial family, and plunge the shogunate into a crisis. Everything will be blamed on Jin and Saki in order to bury the matter. Jin, who is sent to the great jailroom, is subjected to unrelenting treatment by the head of the prisoner’s group and his underlings. Before long, 100 ryo in bribes is paid for Jin by a mysterious man who is described to have the appearance of a merchant. With that bribe, Jin’s life might be spared … … But when he declines an offer by the the head of a prisoner’s group to promote him to one of his attendants and instead dares to ask that everyone’s tatami mats be spread out, the man attempts to instigate the rest of the common criminals to kill Jin. That unexpectedly backfires. Sick of killing people on his orders, the common criminals turn against him. One even strikes him in the chest, and he crumples to the ground unconscious. Guessing that he may have a ventricular tachycardia, Jin thumps hard on his chest and saves his life. In awe, the prisoners reverentially get down on their knees one by one and bow to him. What awaits Jin next is brutal physical torture by government officials. He eventually resigns himself to death and to face Heaven’s judgment. Meanwhile, Katsu, Ryoma and Kyotaro enlist Shinmon Tatsugoro’s cooperation and attempt to intercede with the shogunate to rescue Jin. Although a merchant, Shinmon is close to Tokugawa Yoshinobu because his daughter is one of the shogun’s concubines.

Jin returns to Edo from Nagasaki in disagreement with Ryoma. At that moment, Kyotaro, who has been ordered by his superior to investigate all matters related to Ryoma, visits Jinyudo repeatedly in order to probe. Jin tries once again to recall when Ryoma was assassinated but cannot remember accurately. Then he is overcome by a headache once again. At that moment, a letter addressed to Jin and Saki from Nokaze is delivered. She is to be legally married to Lelong and would like the two of them to come for the wedding ceremony. So Jin and Saki head to Yokohama to attend the wedding, but when they arrive down there, they are told by Nokaze that there is a patient who needs to be examined.

The new government army centred around Saigo enters Edo. Calling themselves ‘Shogitai’, the old government army (retainers of the shogun) that had served the Tokugawa family assemble at Ueno to wait for an opportunity to rise in revolt. Even though Kyotaro has been recommended by Katsu to study in France at a time like this, he agonises over his role in setting in motion Ryoma’s assassination and the path he should continue to take as a vassal of the shogun. Meanwhile, Jin, whose condition has been deteriorating, is asked by Matsumoto to instruct the Institute of Western Medicine at a time when Edo is under a full-scale assault. Aware of the time he has left, Jin tries to pass on his entire medical knowledge to the doctors at Jinyudo. Then the next day finally comes. Kyotaro, who has heard that the new government army will launch an offensive against the old government army, makes a decision.